广东11选5

如何提高RNA提取物的质量和数量

FENLIRNASHI,LIANGHAODESHIYANSHIJISHU**GUANZHONGYAO。YUGUDINGHESHENSHENGDEDNAXIANGBI,RNAGENGBUWENDING。JUYOUHETANGTANGGUJIADERNAHANYOUGAOFANYINGXINGQIANGJI(C-OH)JITUAN,QUEBAOLIANBUDUANZHIZAO,FENJIEHEZHONGFUSHIYONG。FENLIDERNAXUNSUJIANGJIE,HENRONGYIBEIKANGXINGMEIHETANGHESUANMEI(RNases)WURAN,ZHEIXIEHETANGHESUANMEISUICHUKEJIAN。

广东11选5 ZAIBENQIBench BenchZHONG,WOMENCAIFANGLEJIUJINSHANJIAZHOUKEXUEYUANNIAOLEIXUEHEBURUDONGWUXUEXIZHURENJIANCEZHANRENJack DumbacherBOSHI,LEJIETADESHIYANSHIMUQIANDEGONGZUO,JIFENLIZHUANLUZUHEXIAORNAYIJIANDINGHONGJIYINZUXUEBINGDUXULIE。YUBINGDUZUOWEIBINGYUANTIDEDIANXINGGUANDIANXIANGFAN,BINGDUYEKENENGYUSUZHUJUYOUGONGSHENGGUANXIHUOGONGSHENGGUANXI。LEJIENIAOLEIZHONGDEZHEIXIEJINHUAGUANXISHIDumbacherDEYANJIUMUBIAOZHIYI。

广东11选5 “TONGCHANGQINGKUANGXIA,BINGDUKELISHIDIFENGDUDERNA,”DumbacherSHUO。“HETANGTIRNA(rRNA)KENENGSHINI**CHUXUANZEDEHENDAYIBUFEN。WEILEHUODELIANGHAODEHUIFU,WOMENCHANGSHITONGGUOZAITIANJIANCAIJIDAYANGBENBINGJINKENENGXIAOXINDIBAOCUNTAMENLAITIGAORNACHANLIANG。“

优化RNA保存

广东11选5 XUEYUANDESHOUJITANXIANTONGCHANGFASHENGZAIYISHENGWUDUOYANGXINGERWENMINGDEPIANYUANDIQU,RUREDAISENLINHUOSHANHUJIAO。DumbacherTUANDUIMIANLINDEYIDATIAOZHANSHI,YOUYUQUEFALENGZANG,WUFAYONGYEDANKUAISULENGDONGYANGPIN。(RNAZAIYICHUNZHONGWENDINGJIANGJIE,ZHEISHIDNAYANGPINDECHANGJIANFANGFUJI。)ERSHICONGNIAODEXIEZHIQIANG(HOUXIAOHUADAOKAIKOU)CASHIYANGPIN,FANGRURNAWENDINGJIHUOHANYOU4 MNONGDUDELIUQINGSUANGUA(GITC)HUANCHONGYEDEQITAHUNHEWUZHONG。YOUYUYANHANLIANG,SHIYONGYIXIERNAWENDINGJIKENENGNANYIZAIYETIYANGPINZHONGJINXINGRNAFENLI。BAOCHILENGZANGLIANBUSHIWENTI,

确保良好的分离

广东11选5 CHUANTONGSHANG,GITCHUANCHONGYEYIBEIYONGYULIEJIERNATIQUZHONGDEXIBAOHEBINGDUKELI,BINGTONGGUOZANSHISHIRNABIANXINGLAITONGSHIFANGZHIRNaseMEIHUOXING。ZAIZHEIZHONGQINGKUANGXIA,BIXUZAILIEJIEQIANJINXINGYIXIEWANZHENGDEBINGDUFENLIYANGGUOLV,YIBIMIANHESUANTANG。DumbacherTUANDUISHOUJIDEYANGPINZHUYAOSHIYETIXINGSHI - XUYAOHENSHAODEJUNZHIHUA,DANLIANGHAODEFENLIHENZHONGYAO。ZAIXIANCHANG,GAITUANDUISHIYONG0.2μGUOLVQI - LEISIYUXUANZHUANZHUZHONGDEGUOLVQI - LAIGUOLVJIAOXIAODEBINGDUKELI。XIANGWANZHENGXIBAOYIYANGLIUXIAJIAODADEKELI。QITASHIYANSHISHIYONGLENGDONGZUZHIYANGBEN,BIXUKUAISUCHEDIDIJUNZHIHUAYIHUODELIANGHAODEHUIFU。WULUNXUANZEHEZHONGFANGFA,DOUHUIBAOLIUYIDINGLIANGDEJIYINZU(gDNA)。

尽量减少RNase暴露

广东11选5 ZAISHIYANSHIZHONG,XIAOCHUNEIYUANHEWAIYUANRNaseYUCHUNHUARNAZHIJIANDEJIECHUSHIZHONGSHIYIGEWENTI。ZAIRNACHUNHUAGUOCHENGDEZAOQI,RNaseDEWAIBULAIYUANBUNEIMEJUYOUWEIXIEXING。DANSHI,ZAIRNAZHIJIBUZAISHOUGITCRONGYEDENGBIANXINGJIDEBAOHUHOU,RNABEICHUNHUAHOU,NINXUYAOBIMIANJIHUORNases。DANBAIZHIRNaseYIZHIJIKEYIGELISHENGYUDERNase,SHIYONGYUDADUOSHUYINGYONG,TEBIESHINEIXIEHANYOUNEIYUANXINGRNaseDEYINGYONG。ZUOWEIYUFANGCUOSHI,MEIDANGCHUMOSHEBEISHI - YINGGENGHUANBOLIQIMINHUOYIYEQI - SHOUTAO。(YONGYURNACHUNHUADEBOLIQIMINHEQIJUDETUIJIANPEIYANGSHIJIANZAI180°C**200°CSHI**SHAOWEI4XIAOSHI。)XUEYUANDEBIJIAOJIYINZUXUESHIYANSHIJINKENENGSHIYONGDEPCCHULIGUODESHUIDAITIFENZIJISHUI。DEPCSHUITONGGUOJIANJIDEZUANSUANXIUSHISHIRNaseSHIHUO。DANGZAISHINEIZHIZAOSHUISHI,JIANYISHIXIANDUIDEPCSHUIJINXINGGAOYAMIEJUN,YIJIANGJIEDEPC。

增加RNA产量

广东11选5 RUGUONINSHANGWEIZHIDINGTIQU,QINGKAISHIGENZONG。RNACHANLIANGYINBUTONGZUZHIHEYANGPINERYOUHENDACHAYI。DUIYUDumbacher,SHOUJIDEMEIGEYANGPINTONGCHANGRNAHANLIANGDOUHENDI。HUODEDERNALIANGQUJUEYUNIAOLEI**HOUCHIDESHIJIAN,CHIDEDONGXI,YIJIQITAHUAXUEWUZHIHUOXIJUNSHIFOUZAIQIZUOYONG。“JISHIWOMENSHIYONGRNAHANLIANGFEICHANGDIDEYANGBEN,DANGNIGOUJIANNIDEKUSHI,TAHUIFEICHANGLINGRENYINXIANGSHENKE,NIHUIKANDAONIZAILINGYIDUANHUODELEDUOSHAOXULIE,”DumbacherSHUO。(TADEXIAOZUMUQIANZHENGZAISHIYONGXINYIDAICEXUJISHULAIFENLIBINGDUXULIE。)

广东11选5 WEILEZENGJIA(XIANQIANRNAQIANGJIANDE)ZUZHIYANGPINZHONGDERNACHANLIANG,BIMIANGUODUJUNZHIHUAHUOJIARE。YI30MIAODEXIUXIJIANGEYI30MIAODEMAICHONGJUNZHIHUAKEYIGAISHANRNAHUISHOU。CIWAI,SHIYONGERYANGHUAGUIXUANZHUANGUOLVQISHI,YONGGENGDUODESHUIXITUOHUICONGMOZHONGSHIFANGCHUGENGDUODERNA。WULUNNINDESHIYANSHISHIYONGHEZHONGXIAYOUJISHU,CHENGGONGDEFENXIDOUYILAIYULIANGHAODERNAFENLIJISHU。SHIJIANSHI**DE。KUAILEJIEYA!