排列3

凝胶电泳何时用于分离蛋白质?

XUDUORENDOUSHUXISHIYONGNINGJIAODIANYONGFENLIDAFENZI(RUDNA)DEFANGFA。DANSHI,NINGJIAODIANYONGYEKEYIYONGYUFENLIDANBAIZHI。

BUTONGDEDANBAIZHIJUYOUBUTONGDEDAXIAO,ZHUYAOSHIYOUYUANJISUANDANBAIZHISHANGDEQIJIEGOUZHONGGOUJIANDESHULIANG。FUZHEDEHUAXUEXIUSHIYEHUIYINGXIANGQIDAXIAO。BUTONGDEDANBAIZHIYEJUYOUBUTONGDEDIANHE。ZHEIKENENGSHIYOUYUYONGYUGOUJIANTAMENDEANJISUANLEIXINGYIJIYUTAMENLIANJIEDEXIUSHILEIXINGSUOZHI。

BUTONGLEIXINGDEDIANYONGNINGJIAOYONGYUTIGONGBUTONGLEIXINGDEXINXI。YINCI,NINXUANZEDENINGJIAOLEIXINGQUJUEYUNINYAOTIWENDELEIXING。

大小分离

DANBAIZHIDIANYONG

SDS-PAGENINGJIAODIANYONGCONGBUTONGZHONGLEISHAYUDEXUEYEZHONGFENLIDANBAIZHI

TONGCHANG,YOUJUBINGXIXIANANZHICHENGDENINGJIAOGENJUQIBUTONGDAXIAOYONGYUFENLIDANBAIZHI。TONGCHANG,**XIANYAODUIDANBAIZHIJINXINGJIARECHULI,RANHOUZAIJIARUYIZHONGCHENGWEISDSDEHUAXUEWUZHIWEILEYIJIEKAIDANBAIZHI。SDSSHIYIZHONGQUWUJI,KESHISUOYOUDANBAIZHIJUYOUXIANGTONGDEZHENGTIFUDIANHE,YINCI,DANGDIANLIUSHIJIADAONINGJIAOSHANGSHI,FENLIJINSHIYOUYUDANBAIZHIDEDAXIAO。GAIJISHUCHENGWEISDS-PAGE(SDS-JUBINGXIXIANANNINGJIAODIANYONG)。

XIAODANBAIFENZIZAININGJIAOZHONGDEYIDONGBIDADANBAIGENGKUAI,CONGERXINGCHENGYIXILIE“TIAODAI”。MEIGETIAODAIBAOHANTEDINGDAXIAODEDANBAIZHI。ZHEIXIEKEYIYUYIZHIDAXIAODEBIAOZHUNJINXINGBIJIAO。

SDS-PAGENINGJIAOYIYONGYUGENJUDAXIAOFENLIDANBAIZHI。YANGBENSHIGEZHONGSHAYUDEXUEYEZHONG。**YONGDAOBAOHANYIZHIDAXIAODEBIAOJI。DADANBAIZAININGJIAODEDINGBU,XIAODANBAIZAIDIBU。

GAIJISHUKENENGYONGYUXUDUOMUDE,BAOKUOCHUNHUATEDINGDEDANBAIZHI,LIRUFENLIMEISHIPINGONGYEZHONGDEDANBAIZHI。

电荷和pH分离

DENGDIANJUJIAO(IEF)HEQIONGZHITANGNINGJIAODIANYONGSHIDANBAIZHIKETONGGUOQIBUTONGDIANHEFENLIDELIANGZHONGFANGFA。YUSDS-PAGEBUTONG,DANBAIZHITONGCHANGBAOCHIQITIANRAN(ZHEDIE)ZHUANGTAI。SUOYONGNINGJIAODELEIXINGYIJININGJIAOZHOUWEIDERONGYEYEBUTONG。

ZAIQIONGZHITANGNINGJIAODIANYONGZHONG,DANBAIZHIZHUANGZAIZAIKONGDEZHONGJIAN。DAIYOUQIANGFUDIANHEDEDANBAIYI**KUAIDESUDUXIANGNINGJIAODEZHENGCEYIDONG,ERDAIZHENGDIANHEDEDANBAIZEYIXIANGFANDEFANGXIANGYIDONG。

ZHEIZHONGJISHUKEYIYONGYUJUYOUXIANGTONGFENZILIANGDEDANBAIZHIFENKAIZHONGLIANGDANBUTONGDIANHE,HUODANGDAXIAOBINGBUZHONGYAO(LIRUDUIJIBINGDEFAZHANGUOCHENGZHONGZAIBUTONGDEDANBAIZHIDECUNZAIDEBIANHUAKAN)。