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凝胶硬化后，将孔梳移开，并将凝胶放入特殊的电泳槽中。在槽中填充另一种缓冲液，直到有一小层缓冲液完全覆盖琼脂糖凝胶。
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DIANYONGYIZAISHIYANSHIZHONGYOUXUDUOYINGYONG。ZHEILIJINJINSHISHAOSHU：