排列3

电泳如何工作

,TONGCHANGYECHENGWEIDNADIANYONGHUOJIANCHENGWEIDIANYONG,SHIYIZHONGGENJUDAXIAOFENLIDNAPIANDUAN(HEQITADAIDIANFENZI)DEJISHU。TONGCHANGSHIYONGQIONGZHITANGNINGJIAOHEDIANHEWANCHENGCIBUZHOU,YISHIPIANDUANBICIFENLI。

GAIJISHUJUYOUXUDUOYINGYONG,BAOKUOJIANCHADNA,DNAZHIWEN,FANZUIXUEYIJIGEZHONGYILIAOYINGYONG。

什么是凝胶电泳?

NINGJIAODIANYONGSHIYIXIANGJISHU,KERANGKEXUEJIAGENJUDAXIAOFENLIDAIDIANFENZI。ZHEIBAOKUODNA,RNAHEDANBAIZHI。

QINGJIZHU,YOUYUFENZIDETANG-LINSUANGUJIAZHONGDAIFUDIANDEYANGFENZI,DNAHERNADAIYOUQINGWEIDEFUDIANHE。QUJUEYUDUOTAILIANZHONGDEANJISUAN,DANBAIZHIKEYIJUYOUYIDINGFANWEIDEDIANHE。

电泳的组成

WEILEJINXINGDIANYONG,**XIANXUYAOZHIBEININGJIAO。ZHEIJIHUKEYIZAIRENHESHIYANSHIZHONGWANCHENG。QIONGZHITANGNINGJIAOSHI**CHANGJIANDE。WEILEZHIZUONINGJIAO,JIANGQIONGZHITANGFENYUYIZHONGCHENGWEIDIANYONGHUANCHONGYEDETESHUHUANCHONGYEHUNHE。RANHOUJIAREGAIHUNHEWUZHIZHIQIONGZHITANGRONGJIEBINGZAIHUANCHONGRONGYEZHONGCHONGFENHUNHE。

QINGZHUYI,MOUXIEDIANYONGFANGANYAOQIUTIANJIAXIUHUAYIDING(Et-Br)。ZHEIHUIRANSEDIANYONGZHONGSHIYONGDESUOYOUDNA,YISHININKEYIZAIZIWAIXIANXIACHAKANPIANDUANDEWEIZHI。

塑造凝胶

RANHOUJIANGQIDAORUCHENGWEININGJIAOJIAOZHUTUOPANDEJUXINGMOJUZHONG。YUCHANSHENGYONGYUDIANYONGDEJUXINGNINGJIAODEMOJUYIQI,JIANGSHUZIZHIYUNINGJIAODEYIDUAN。ZHEIZHONGSHUZIKEYIJIANGNINXIWANGTONGGUODIANYONGFENLIDEYANGPINZHUANGZAIDAOKONGZHONG。NINKEYIZAIZHEILIKANDAOTUPIAN。

凝胶硬化后,将孔梳移开,并将凝胶放入特殊的电泳槽中。在槽中填充另一种缓冲液,直到有一小层缓冲液完全覆盖琼脂糖凝胶。

GAIGUANTONGGUOHUANCHONGRONGYE,ZAITONGGUOQIONGZHITANGNINGJIAOCHANSHENGDIANLIU(FANWEIWEI50ZHI150 V)。QIONGZHITANGNINGJIAODEKONGWEIYUDIANLIUDEFUJI(YINJI),NINGJIAODELINGYIDUANWEIYUDIANLIUDEZHENGJI(YANGJI)。

电泳如何工作?

ZAIDIANLIUTONGGUOSHUIXIANGHENINGJIAOZHIQIAN,NINDEYANGPINYIZHUANGRUKONGZHONG。ZHEISHISHIYONGWEILIANGYIYEQIWANCHENGDE。“BIAOJI”YANGBEN(YECHENGWEIDNAJIETI)SHIJUYOUYIZHIDNAPIANDUANDAXIAODEYANGBEN,KEYIBANGZHUNINBIJIAOYANGBENBINGLEJIESUOCESHIYANGBENDEDAXIAO。

TONGCHANG,JIANGGENZONGRANLIAO(YECHENGWEITIANCHONGRANLIAO)TIANJIADAOMEIGEYANGPINZHONG,YIBANGZHUNINJIANGYANGPINJIAZAIDAOKONGZHONG。RANLIAOHAIKEYIBANGZHUNINGENZONGYANGPINZAININGJIAOZHONGDEYUNDONG。

NEIMEYANGPINSHIJISHANGRUHEZAININGJIAOZHONGYIDONGBINGANDAXIAOFENKAI?TAYULIUGUOQIONGZHITANGNINGJIAODEDIANLIUYIJIPIANDUANHEQIONGZHITANGNINGJIAODEDAXIAO/JIEGOUYOUGUAN。

电荷和大小决定DNA条带

QINGJIZHU,ZONGTIERYAN,DNADIANHESHIFUDE。YINCI,DANGJIANGZHEIXIEYANGPINFANGZHIZAIKAOJINDIANLIUFUDUANDEKONGZHONGSHI,ZHEIJIANGDAOZHIDAIFUDIANHEDEDNA CONGYINJIYIZOU(FUDIANHE),BINGZAIXIANGFANDUANYIXIANGYANGJI(ZHENGDIANHE) 。

CHULEYANGPINDEZHEIZHONGYIDONGZHIWAI,DIANYONGHAITONGGUODAXIAOJIANGYANGPINHEZHEIXIEYANGPINZHONGDEPIANDUANFENLI。ZHEISHIYINWEIJIAOXIAODEFENZIHESUIPIANKEYIGENGKUAI,GENGQINGSONGDITONGGUONINGJIAO,ERJIAODADEFENZIHESUIPIANZEYIDONGGENGMAN。ZHEIYIWEIZHEXIAOPIANDUANJIANGBIDAPIANDUANGENGKUAIDIYIZHININGJIAOMODUAN,YINCI,MEIGEPIANDUANJUNANDAXIAOFENKAI。

NINGJIAOYUNXINGYUEYIGEXIAOSHI(ZAIDADUOSHUGUICHENGZHONG)HOU,GUANBIDIANHEBINGFENXININGJIAO。NINHUIZAININGJIAODEGEGEDIANKANDAOYIGEMINGXIANDEJUXINGDAI,TONGCHANGCHENGWEIDNADAIHUODANBAIZHIDAI。MEIGEDAIDAIBIAOYIGEYANZHENINGJIAOYIDONGDEPIANDUAN。

电泳仪的应用与用途

DIANYONGYIZAISHIYANSHIZHONGYOUXUDUOYINGYONG。ZHEILIJINJINSHISHAOSHU:

  • 用于犯罪现场和基因检测的 DNA指纹
  • 测试聚合酶链反应产物
  • 用于医学目的的基因分析
  • 在物种或后代之间比较DNA
  • 分析物种之间的进化和分类学关系
  • 了解限制酶在哪里切割DNA的不同部分
  • 亲子鉴定
  • 测试抗生素耐药性