排列3

凝胶电泳实验室程序



凝胶电泳是实验室中用于测量和分类DNA链的一种方法。这是必需的,因为即使在大多数显微镜下观察,正常条件下的DNA仍然很小,无法操作。凝胶电泳实验室使用相对简单的程序,同样的基本技术也可以用于分离单个蛋白质。

凝胶基质

YAOKAISHININGJIAODIANYONGCHENGXU,NIN**XIAN**CHUANGJIANNINGJIAO。TONGCHANG,SHIYONGCHENGWEIQIONGZHITANGDEWUZHIJIANGNINGJIAOZHICHENGBOPIAN。JIANGQIONGZHITANGFENFANGRUSHAOPINGZHONG,RANHOUFANGRUCHENGWEIHUANCHONGYEDEYANSHUIZHONG。JIAREQIONGZHITANGHEHUANCHONGYEDEHUNHEWU,ZHIDAOLIANGZHONGWUZHIRONGHUAZAIYIQI,RANHOUDAORUCHENGXINGMOJUZHONG。RANHOUZAININGJIAOLENGQUEZHIQIAN,JIANGYIZHONGCHENGWEISHUZIDEZHUANGZHIFANGZAIMOJUDEYIDUAN。NINGJIAOLENGQUEHOU,JIANGSHUZIYIKAI,ZHILIUXIAHENXIAODEFENGXI,KEYONGLAIGUDINGDNAYANGPIN。

LENGQUEDEQIONGZHITANGHUNHEWU(CHENGWEININGJIAOJIZHI)DETESHUTEXINGSHIYOUYUTASHIYONGYANSHUIZHICHENGDE。TONGDIANHOU,JITIJIANGBIANDEDAODIAN,CONGERYUNXUDIANLIUYANQIZHANGDULIUDONG。NINGJIAOJIZHIDELINGYIGETESHUXINGNENGSHIGUIZEDEWEIGUANKONGDECUNZAI。ZHEIXIEKONGJIANGSHIDNALIANCHUANGUONINGJIAOJIZHIBINGCUJINFENXUANGUOCHENG。DIANYONGSHI

XIAYIBUSHICHUANGJIANYIGEDIANYONGSHI。ZHEISHIYIGEJUXINGDEXIAOHEZI,LIANGDUANDEZHENGJIHEFUJIDIANQILIANJIE。QIANGSHITONGCHANGSHIQIANDE,ZUGOUXIAOYISHIHEZHUOMIAN,BINGYOUYOUJIBOLIDENGTOUMINGCAILIAOZHICHENG。

JIANGYANSHUIDAORUDIANYONGSHIDEDIBU,NINGJIAOJIZHILVEWEIJINRUGAIRONGYEZHONG。YANSHUIYOULIANGGEZUOYONG:BANGZHUDIANLIULIUDONGHEBAOCHININGJIAOJIZHISHIRUN。YOUYUDNAYOUFUDIANHETUIDONG,YINCIFANGZHIJIZHI,SHIYANGPINWEIYUFUDIANLIANJIEPANGBIAN。

制备DNA

RANHOUZHIBEIDNAYANGPIN。YOUYUJIHUKANBUDAORONGYEZHONGDEDNA,YINCIJIANGYIZHONGCHENGWEIJIAZAIHUANCHONGYEDEZHESEJITIANJIADAOMEIGEDANDUDEYANGPINZHONG。GAISHIJIHAIKEYISHIDNARONGYEZENGCHOU,SHIQILIUDONGXINGGENGXIAO,GENGKEXING。SHIYONGYIYEQIJIANGDNARONGYEYANGPINZHUANYIDAONINGJIAOJIZHIDEMEIGEJIAOTICAOZHONG。ZAIMEIGEYANGPINZHI​​JIANDEKONGCAOZHONG,FANGZHIYIXIEZHANGDUYIZHIDEDNARONGYE(CHENGWEIDNABIAOZHUNPIN),YONGYUSHIYANKONGZHIHEBIJIAO。

打开电源

XIANZAI,DAKAIDIANYONGSHI。ZAIFUDIANYUANXIA,NINDEDNAYANGPINJIANGBEIPOCHUANGUOPEIYANGSHIDEZHENGGEZHANGDU。XIAODNALIANJIANGGENGKUAIDITONGGUONINGJIAOJIZHI,BINGQIEZAIDUANSHIJIANNEITAMENHUIYUJIAOZHANG,JIAOMANDELIANFENLI。ZHESEJIZHONGDERANLIAOKERANGNINZHUIZONGDNA。NINJIANGKANBUDAODNADEGEGELIAN,DANSHIXIANGTONGZHANGDUDELIANHUIJUZAIYIQI。

**后步骤

FENLICHUDNAHOU,CONGDIANYONGSHIZHONGQUCHUJIZHI。RANHOUJIANGDNARANSE,YIBIANYUCELIANGHEJIANCHA。