排列3

什么原因导致电泳涂片?



您是否想知道科学家如何研究像您的DNA这样的微小片段?一种方法是凝胶电泳。电泳通常会产生清晰易读的条带,非常适合科学解释,但涂片结果有时会掩盖数据。

TL; DR(太长;未读)

NINGJIAODIANYONGSHIKEXUEJIANENGGOUKESHIHUAXIAOHUADEYANGPINBINGCELIANGPIANDUANDEDAXIAO。TUMOBUDANGSHIYOUYUQIONGZHITANGNINGJIAOZHIBEIBUDANG,WEIXISHIYANGPINZHUANGRUKONGZHONGHUOSHIYONGLIEZHIYANGPINZAOCHENGDE。

什么是电泳?

NINGJIAODIANYONGSHIKEXUEJIAKESHIHUAXIAOFENZI(LIRUDNA)DEXIAOHUAYANGPINBINGGUSUANZHEIXIEPIANDUANDAXIAODEYIZHONGFANGFA。WEILEJINXINGDIANYONG,KEXUEJIATONGGUOJIANGQIONGZHITANGXUANFUZAIFEISHUIZHONGLAIZHIBEININGJIAO。SUODEDEJUHEFANYINGCHANSHENGLEZONGHENGJIAOCUODETANGJUHEWU,YINCIGAININGJIAOZAIHUAXUECENGMIANSHANGKANQILAIYOUDIANXIANGZHIZHUWANG。

KEXUEJIASHIYONGQIEGEYIQIZAININGJIAOZHONGXINGCHENGKONG,ZHEIYANGTAMENJIUKEYIJIANGFEICHANGSHAOLIANGDEXIAOHUAYANGPINJIAZAIDAOKONGZHONG。KAIQIJIQIHUISHIDIANNENGLIUGUONINGJIAO,BINGQIEYANGPINZHONGDESUIPIANKAISHICONGKONGZHONGXINGJINDAONINGJIAODELINGYICE。YOUYUNINGJIAOSHIWANGZHUANGDE,JIAOXIAODESUIPIANHUIKUAISUCHUANGUOJIZHI,ERJIAODADESUIPIANZEXUYAOGENGZHANGDESHIJIANCAINENGCHUANGUOJIZHI。WANCHENGHOU,NINGJIAOZHONGDEHEIDAIDAIBIAOBUTONGPIANDUANDECHUANBOJULI。KEXUEJIACELIANGZHEIXIETIAODAI,BINGSHIYONGDUISHUJISUAN,GENJUQIQIANYIDEJULIQUEDINGMEIGESUIPIANDEDAXIAO。

KEXUEJIAXIWANGYOUQINGXIDETIAODAI,DANYOUSHITIAODAIHUITUPIAN。ZHEIZHONGTUMOTONGCHANGSHIYOUYUNINGJIAOZHIBEIBULIANG,WEIXISHIDEYANGPINSHANGYANGZHIKONGZHONGHUOZHILIANGJIAOCHADEYANGPINSUOZHI。

凝胶准备不理想

DANGSHEJITUMOJIEGUOSHI,YIGEKENENGDEZUIKUIHUO**SHIZHUNBEIBUCHONGFENDENINGJIAO。LINGRENMANYIDENINGJIAOJUNYUNJUHE,ZAILIUYANPANZHONGDEZHENGGENINGJIAOZHONGCHANSHENGJUNYUNDEJIZHI。RUGUOYIBUFENNINGJIAO(TONGCHANGSHIXIABANBUFEN)ZAIKEXUEJIAWANCHENGZHENGGETUOPANQINGDAOZHIQIANNINGGU,ZESHENGCHENGDENINGJIAOJIANGBUJUNYUNBINGCHANSHENGTUMODEJIEGUO。

样本过多

ZAIJIANGYANGPINJIAZAIDAOKONGZHONGZHIQIAN,**JIANGZHEIXIEYANGPINXISHIDAOZUYITONGGUONINGJIAODECHENGDU,ERBUHUIYICHUKONG。RUGUOYOUYUKEXUEJIAWANGJIXISHIYANGPINHUOSHIYONGLEBUSHIDANGDEXISHIYINZIERSHIYANGPINNONGDUGUOGAO,NEIMESUIPIANDUIYUKONGLAISHUOJIANGTAIDAERCHANSHENGWUDIAN。

劣质样品

BULIANGDEYANGPINZHILIANGYEHUIZAOCHENGTUOWEIXIANXIANG。LIRU,BEIDANBAIZHIWURANHUOHANYANGUODUODEDNAYANGPINKENENGHUICHANSHENGTUOYING。JIANGJIEHUOBIANXINGDEYANGPINYEHUICHANSHENGJIAOCHADEJIEGUO,BAOKUOTIAODAINONGZANG。

NINGJIAODIANYONGSHIKEXUEJIAGUANCHAXIAOHUAHOUDEYANGPINBINGQUEDINGPIANDUANDAXIAODE**JIAFANGFA。JINGXINZHUNBEININGJIAOHEYANGPIN,KE**DACHENGDUDIJIANSHAOTUMODEKENENGXING,BINGCHANSHENGQINGXIDETIAODAI,SHIKEXUEJIESHIDELIXIANGZHIXUAN。