排列3

电泳的目的

DIANYONGSHIYIZHONGGONGNENGQIANGDAQIEBIANYIDEFENZIFENLIJISHU。CUNZAIJINXINGDIANYONGDEGEZHONGYUANYIN,BAOKUOYUFENZIDEFEIQINRUXINGJIEHEHEFENZIFENLIDEKESHIHUA。ZONGTIERYAN,DIANYONGDEMUDESHITIGONGYIZHONGFENXIWUZHIDEZHUNQUEFANGFA,LIRUNINDEXUEYEHEDNA(TUOYANGHETANGHESUAN),ZHEIXIEWUZHIHENNANYONGCHANGGUIFANGFAFENLI。

定义

DIANYONGSHIYIZHONGJINGYANJISHU,YONGYUGENJUDIANLIUZHONGDEXIANGYINGFENLIZHURUXIBAOHEDANBAIZHIDENGDAIDIANFENZI(ZHENGFU)。

YINGXIANGDIANYONGDEYINSUYOUHENDUO,BAOKUOJINGDIANHE,FENZIZHILIANG,HUANCHONGYEHEDIANYONGJIEZHI,RUZHIHUONINGJIAO。ZAIDIANYONGZHONG,FENZIXIANGXIANGFANDEDIANHEYIDONG;LIRU,JUYOUZHENGJINGDIANHEDEDANBAIZHIHUIXIANGDIANYONGJIEZHIDEFUJIYIDONG。CIWAI,ZHILIANGJIAOXIAODEFENZIBIZHILIANGJIAODADEFENZIYUNDONGGENGKUAIHUOFENLIGENGKUAI。

历史

1937NIAN,YIWEIMINGWEIArne TiseliusDERUIDIANKEXUEJIAKAIFALEYIZHONGYONGYUCELIANGDANBAIZHIFENZIYUNDONGDEYIQI,CHENGWEI“YIDONGBIANJIEYIQI”。ZHEISHIYIZHONGSHIYONGSHUIJIEZHIFENLIDANBAIZHIFENZIDEUXINGSHEBEI。

1940NIAN,YINRULEQUYUDIANYONGFA,GAIFANGFASHIYONGGUTIJIEZHI(LIRUNINGJIAO),BINGYUNXURANSEYISHIXIANGENGHAODEFENBIANLVHUOKESHIHUAFENZIFENLI。

RANHOUZAI1960NIAN,MAOXIGUANDIANYONGJISHUDEYIFAZHAN,YITIGONGYIZHONGTONGYONGDEDIANYONGJISHU。ZHEIZHONGDIANYONGYUNXUSHIYONGSHUIXINGHEGUTAIJIEZHIFENLIFENZI。

分子结合

SHIYONGJIEZHIDEDIANYONGYOUYIYIFEIQINRUXINGFANGSHIYUFENZIXIANGHUZUOYONG。LIRU,NINGJIAOJIEZHIYUDANBAIZHIFENZIJIEHEERBUHUIPOHUAIDANBAIZHIDEJIEGOUHEGONGNENG。YUFENZIJIEHEHOU,TONGGUOSHIJIADIANLIUKAISHIYIDONGHUOFENLI。CIWAI,YEKEYIZAIDIANYONGHOUHUISHOUJIEHEZHIPEIYANGJIDEFENZI。

高分辨率分离

电泳仪旨在可视化分子的分离。这可以通过各种方法来实现,包括染色和放射自显影。

FANGSHEZIXIANYINGSHIYONGXSHEXIANJIAOPIANZAIFENLIHOUKESHIHUAFANGSHEXINGFENZI(LIRUDNA)DEWEIZHI。ZHEIZHONGLEIXINGDEKESHIHUAXIAOGUOKEYUPAIZHAOXIANGPIMEI,QIZHONGXSHEXIANJIUXIANGZHAOXIANGJISHANGUANGDENG,ERXSHEXIANJIAOPIANXIANGSHIYONGYUCHONGXIHEIBAIZHAOPIANDEJIAOPIAN。ZAIDIANYONGZHONG,XUEYEZHONGDEDANBAIZHIDENGFENZIDEZHAOPIANSHISHIYONGFANGSHEZIXIANYINGJISHUXIANYINGDE。

ZAIRANSEZHONG,ZAIFENLIGUOCHENGZHIQIANHUOZHIHOU,JIANGZHURUKAOMASILANHEXIANANHEIDERANLIAOYUFENZIHUNHE。LIRU,ZAIDIANYONGQIANJIANGDANBAIZHIYUKAOMASIRANLIAOHUNHEHUICHANSHENGRANSEDELUJING(XIAODIANHUOXIAN),XIANSHIDANBAIZHIZAIFENLIGUOCHENGZHONGDEYUNDONG。

定量分析

DIANYONGYIDELINGYIGEMUDESHIZAIKESHIHUAFENZIFENLIHOUHUODEDINGLIANGXINXI。WEILEHUODEDINGLIANGSHUJU,LIRU,TUXIANGFENXIRUANJIAN(2DHE3DXUANRANRUANJIAN)JIANGDIANYONGJIEGUOJILUWEISHUZIXINHAO。ZHEIXIEXINHAODAIBIAOFENZIZAIDIANYONGZHIQIANHEZHIHOUDEWEIZHI,RANHOUBEIYONGYU“JISUANJI”DINGLIANGFENXI(SHIYONGJISUANJI)。