排列3

DNA样品如何收集和准备进行研究



在对DNA进行测序或通过基因工程对其进行改变之前,科学家****先对其进行分离。这似乎是一项艰巨的任务,因为细胞包含多种其他化合物,例如蛋白质,脂肪,糖和小分子。幸运的是,生物学家可以利用DNA的化学特性从这些污染物中分离出DNA,并为进一步研究做准备。此过程称为DNA提取。

细胞裂解

YOUXUDUOBUTONGDEJISHUYONGYUDNATIQU。DANGESHIYANSHISUOSHIYONGDEDNAQUJUEYUYAOJINXINGDESHIYANLEIXINGYIJIDNADECHUNDU。KEXUEJIATONGCHANGCONGHANYOUXIBAODEYANGBENKAISHI,LIRUZUZHIHUOXUEYEYANGBEN,RANHOUJIANGXIBAONONGKAIHUOLIEJIE。NINKEYITONGGUODUOZHONGFANGSHILIEJIEXIBAO。TIANJIAQINGJIEJIHUISHITAMENSANKAI,BINGSHIQIJINGSHOUGAOPINSHENGBO。HUOZHE,JIANGYANGPINYUBOLIZHUHUNHEBINGSHIQIKUAISUZHENDONG,HUIWULIFENLIEXIBAOBINGSHIFANGQINEIRONGWU。

快速和肮脏的方法

RUGUOBUXUYAOGAOCHUNDU,KEXUEJIAKEYITIANJIAYIZHONGCHENGWEIDANBAIMEIKDEMEILAIFENJIEYANGPINZHONGDEDADUOSHUDANBAIZHI,RANHOUZHIJIESHIYONG。DANSHI,YOUYUDADUOSHUWURANWURENGRANCUNZAI,YINCIGAIJISHUFEICHANGANGZANG,YINCIJINZAIYOUXIANKAOLVSUDUQIECHUNDUBUCHENGWENTIDEQINGKUANGXIACAISHIYONG。LINGYIZHONGKUAISUERANGZANGDEFANGFASHITONGGUOTIANJIAYAN(RUANYANHUOYISUANJIA)YIPOSHIDANBAIZHICHENDIANLAITIGAOYANNONGDU,CONGERQUCHUDANBAIZHI。YOUYURENGRANCUNZAIXUDUOQITAWURANWU,YINCIGAIJISHUYEXIANGDANGZANG。

苯酚-氯仿萃取

LINGYIZHONGFANGFASHIYONGQUWUJIRONGJIEXIBAO,RANHOUJIANGRONGYEYUYIWUCHUN,LVFANGHEBENFENHUNHE。RANHOUJIANGRONGYEFENWEILIANGCENG。DANBAIZHI**ZHONGBAOLIUZAISHANGBUYOUJICENGZHONG,ERDNABAOLIUZAIXIABUSHUICENGZHONG。CIJISHUXUYAOZIXIKONGZHIYANNONGDUHEpHCAINENGHUODELIANGHAOJIEGUO。ZHEIHENHAOSHI,ERQIEBENFENHELVFANGDOUSHIJUDUHUAXUEPIN。YINCI,SUIRANBENFEN-LVFANGCUIQUCENGJINGSHICHANGGUICAOZUO,DANJINNIANLAIQITAJISHUYEBIANDEYUELAIYUELIUXING。

阴离子交换色谱

YUBENFEN-LVFANGCUIQUXIANGBI,YINLIZIJIAOHUANSEPUFAJUYOUGENGGAODECHUNDUHEGENGYIZHIDEJIEGUO。GUANHUOZHUZHONGZHUANGYOUXIAOKELI,ZHEIXIEXIAOKELISHANGDAIYOUDAIZHENGDIANHEDEWEIDIAN,DAIFUDIANHEDEFENZIHUOYINLIZIKEYIYUZHIJIEHE。DNAYUZHEIXIEYINLIZIJIAOHUANWEIDIANJIEHE,ERQITAWURANWU(RUDANBAIZHIHERNA)ZECONGSEPUZHUSHANGXIDIAO。ZHIHOU,SHIYONGFUHANYANDERONGYEJIANGDNACONGSEPUZHUZHONGLACHU。

套件

CHUNHUADNA**KUAI,YEXU**KEKAODEJISHUSHISHIYONGTEZHISHIJIHE。ZHEIXIETAOJIANZAISHIGUANZHONGBAOHANGUIJIAOMO。DNAHUIZHANFUZAIMOSHANG,TONGSHISHIYONGSHIJIHESUIFUDEYIXILIEZHUANMENZHUNBEIDEYANRONGYEJIANGQITAWURANWUXIDIAO。**HOU,YONGDIYANRONGYECONGZHUZISHANGXIDIAODNA。ZHEIXIESHIJIHEKUAISU,YIYUSHIYONG,BINGTIGONGKEZHONGFUDEJIEGUO。

吸光度

YIDANFENLICHUDNABINGJIANGQIZHONGXINXUANFUZAIpHKONGZHIDEHUANCHONGRONGYEZHONG,**HOUYIBUJIUSHICESHIQICHUNDU。YIZHONGJIANDANFANGBIANDEFANGFASHIJIANCHATAZAI260HE280NAMIBOZHANGCHUXISHOULEDUOSHAOZIWAIXIAN。RUGUODNASHICHUNJINGDE,ZE260NAMIDEXIGUANGDUCHUYI280NAMIDEXIGUANGDUYINGDENGYU1.8。TONGGUOCELIANG260NAMIDEXIGUANGDU,NINHAIKEYIQUEDINGDNADENONGDU。