排列3

什么是蛋白质印迹测试?



Western blotCESHI,YECHENGWEI**YINJI,SHIDUIDANBAIZHIHUNHEWUZHONGTEDINGDANBAIZHIDECESHI。DANBAIZHIYINJISHIYANSHIZAININGJIAODIANYONGHUOMEILIAN**XIFUCEDING(ELISA)SHIYANHOUJINXINGDE,TASHIYONGKANGTILAIJIANDINGTEDINGDEDANBAIZHI。

SDS页面

SHIERWANJILIUSUANNAJUBINGXIXIANANNINGJIAODIANYONG(SDS-PAGE)SHIYONGYUFENLIDANBAIZHIYIJINXINGDANBAIZHIYINJIDECHANGYONGCESHI。DANGDANBAIZHITONGGUONINGJIAOJIZHISHI,DANBAIZHIANZHONGLIANGHEDIANTEXINGFENKAI。

酶联**吸附

ELISACESHISHIYONGFUZHEZAIGUTIBIAOMIANSHANGDEMEIHUOKANGTILAICHUANGJIANCESHIBIAOMIAN。RANHOUJIANGYANGPINTIANJIADAOCESHIBIAOMIAN。YUDANBAIZHIFANYINGHUOFUZHEDEKANGTIHUOMEIXIANSHIYANGXINGJIEGUO。

蛋白质印迹

ZAININGJIAODIANYONGHOUJINXINGDANBAIZHIYINJICESHI。FENLICHUDEDANBAIZHIBEIZHUANYI(HUOYINJI)DAOXIAOSUANXIANWEISUMOHUONILONGMOSHANG,BINGTONGGUOBEIERJIDANBAIZHIBIAOJIDETEYIXINGKANGTIJINXINGJIANDING。

正面测试确认

WesternYINJICESHIYONGYUQUERENNINGJIAODIANYONGHUOELISACESHIDEYANGXINGJIEGUO。Western blotJIANCEKEYIGENGTEYIXINGDIJIANDINGDANBAIZHI,BINGKEYIPAICHUJIAYANGXING。

疾病

Western blotJIANCETONGCHANGYONGYUQUERENRENLEI**QUEXIANBINGDU(HIV)HELAIMUBINGDEYANGXINGJIANCEJIEGUO。