排列3

为什么在DNA提取中使用钠?



DNA(脱氧核糖核酸)是细胞核内包含遗传信息的分子。提取DNA涉及一系列步骤,以轻轻地打开细胞,打开核膜,从蛋白质中分离出DNA,然后使其从溶液中沉淀出来。根据膜的结构,DNA及其电负性,使用各种化学物质即可完成此操作。脱除蛋白质后,可使用氯化钠或其他含钠化合物稳定DNA,并有助于沉淀。

DNA的结构

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DNA极性

“JIXING”SHIHUAXUESHUYU,MIAOSHULEBAOHANBUJUNYUNDIANHEFENBUDEFENZI。GENJUKANGNAIERYIXUEYUANDEPaul ZumboSUOSHUO,SUOYOUHESUANDOUSHIJIXINGDE。DUIYUDNA,GUJIASHANGDEGAOJIXINGLINSUANJITUANDAIYOUFUDIANHE。GAIXINGZHIJIESHILESHUIRONGXING,YINWEISHUIYESHIJIXINGDE。SHUIDEZHENGDIANHEYUDNADEFUDIANHEXIANGHUZUOYONG,XINGCHENGRONGYE。WEILEHUISHOUDNAYIJINXINGJINYIBUCESHIHUOKESHIHUA,**YONGSHUIJIANGDNACONGRONGYEZHONGCHENDIANCHULAI。YOUYUSHUIJUYOUXIANGDUIRUODEZHENGDIANHE,YINCIKEYITONGGUOZAIRONGYEZHONGTIGONGGENGQIANGDEDAIZHENGDIANDELIZILAISHIXIAN。NASHI**LIXIANGDEXUANZE。

使用钠和酒精沉淀DNA

YIDANDNACONGXIBAOHEZHONGYICHUBINGYUSHUIHUNHE,NALIZIDEYINRUJIUHUIZAINAHEGUJIAZHIJIANCHANSHENGZANSHIDEXIYINLI。DNABEIZANSHIZHONGHE,RANHOURONGYICONGSHUIZHONGJIELI。ZAIZHEIYIJIEDUAN,YOUYUJIUJINGSHIFEICHANGFEIJIXINGDE,YINCIYINRUJIUJINGPOSHIDNAHENALIZIZHIJIANDEJIEHEGENGJIAJINMI。KEYISHIYONGYICHUNHUOYIBINGCHUN。YIDANDNACONGSHUIZHONGJIELIBINGYUNAJINMIJIEHE,TAJIANGCONGRONGYEZHONGCHENDIANCHULAI,KEYIJIANGQINONGSUOYIJINXINGCHUNHUA,YEKEYITONGGUOJIANGQIQINGQINGDIRAOZAIGUANGHUADEBOLIBANGSHANGJINXINGKESHIHUA。

DNA提取的其他步骤

FENJIEZHIMOHEHEMOYICONGXIBAOZHONGHUOQUDNATONGCHANGSHITONGGUO**XIANYINRUMOUZHONGQUWUJILAIFENJIEZHIZHIFENZILAIWANCHENGDE。SHIYANSHIZHONGCHANGYONGDEXIDIJISHISDSHUOSHIERWANJILIUSUANNA。DANSHIDUIYUJIANDANDETIQU,SHENZHIKEYISHIYONGXIJIEJING。RUGUOXIBAOLAIYUANYUZHIWUCAILIAO,TONGCHANGHAITIANJIAMEILAIXIAOHUAXIBAOBI。