排列3

蛋白质印迹法的缺点

DANBAIZHIYINJISHISHENGHUASHIYANSHIZHONG**CHANGJIANDECHENGXUZHIYI。JIBENSHANG,TACONGDAXIAOSHANGJIANGDANBAIZHIYUYANGPINFENKAI,RANHOUSHIYONGKANGTIJINXINGCESHIYIQUEDINGSHIFOUCUNZAIJIDINGDEDANBAIZHI。TABUJINZAIYANJIUZHONGYOUYONG,ERQIEZAIYIXUEHUOZHENDUANSHIYANSHIZHONGYEYOUYONG。LIRU,ZHENDUIHIVHELAIMUBINGDEJIANCEJUNBAOKUOMEILIAN**XIFUCEDING(ELISA)JIANCE,RUGUOELISAJIANCECHENGYANGXING,ZEJINXINGDANBAIZHIYINJIFENXI。RANER,JINGUANTAHENLIUXING,DANSHIDANBAIZHIYINJIJUYOUYIXIEQUEDIAN。

非量化

GUDIANDANBAIZHIYINJISHIFEIDINGLIANGDE。HUANJUHUASHUO,JINGUANTAMENKEYIGAOSUYANJIURENYUANSHIFOUCUNZAIMOUZHONGTEDINGDEDANBAIZHI,DANTAMENWUFALIANGHUACUNZAIDUOSHAODANBAIZHI。YIXIESHENGWUJISHUGONGSIXIANZAICHUSHOUSHIJIHE,SHIYANJIURENYUANHUOSHIYANSHIJISHURENYUANNENGGOUSHIYONGBIAOZHUNQUXIANDINGLIANGDANBAIZHIDEHANLIANG-DANZHEIJINZAIYOUXIANGTONGDANBAIZHIDECHUNYANGPINKEYONGSHICAIYOUXIAO。CIWAI,DANBAIZHIDEFENZILIANGZHINENGTONGGUODANBAIZHIYINJIFAGUSUAN,ERBUNENGXIANGZHIPUFANEIYANG**QUEDING。

抗体

JINDANGKEHUODEZHENDUIMUDEDANBAIDEYIKANGSHI,CAINENGJINXINGDANBAIZHIYINJI。SHENGWUJISHUGONGSIKEYITIGONGXUDUOBUTONGDANBAIZHIDEKANGTI,DANTAMENBINGBUBIANYI;RUGUOWUFASHIYONGJIDINGDANBAIZHIDEYIKANG,ZEWUFAJINXINGDANBAIZHIYINJIYIXUNZHAOTEDINGDANBAIZHI。CIWAI,YANJIURENYUANKENENGXIANGQUEDINGMOUZHONGDANBAIZHISHIFOUYIBEIXIUSHI(LIRU,SHIFOUYIBEILINSUANHUA(LIRU,QISHANGYOUYIGELINSUANJITUANLIANJIE)),BINGQIETONGGUODANBAIZHIYINJIJISHU,TAMENXUYAODUIGAIXIUSHIDANBAIJUYOUTEYIXINGDEKANGTIDANBAI。

训练

正确进行蛋白质印迹并获得良好的结果可能具有挑战性,因此**对员工进行良好的培训。在这方面,经验也许是**好的导师。即使对于有经验的技术人员,蛋白质印迹也是费时的。例如,实验的凝胶电泳仪部分通常需要一到两个小时才能运行。当然,在凝胶运行时还可以执行其他任务,但是实验仍然需要相当长的时间才能获得结果。

其他限制

KANGTIYOUSHIKENENGHUIBIAOXIANCHUTUOBAJIEHE,ZHEIKENENGDAOZHIJIAOCHADEJIEGUO。CIWAI,TONGGUODANBAIZHIYINJI,NINJIANGSHIYONGZHENDUITEDINGDANBAIZHIDEKANGTI,YINCININDEJIEGUOZHIHUIGAOSUNINGAIDANBAIZHISHIFOUCUNZAI。XIANGBIZHIXIA,GAOFENBIANLVZHIPUFENXIJIESHILEYANGPINZHONGCUNZAIDESUOYOUDANBAIZHI,YUJINGDIANDEDANBAIZHIYINJIBUTONG,TASHIDINGLIANGDE。DANGRAN,ZHONGYAODESHIYAOJIZHU,YUDANBAIZHIYINJIFAXIANGBI,ZHIPUFAANGGUIDEDUO,BINGQIESHIYONGQILAIZAIJISHUSHANGYEGENGJUTIAOZHANXING。