排列3

如何阅读蛋白质印迹



Western印迹是临床医生可能会使用或要求的一种诊断技术。Western印迹通过分离样品(通常是血液样品)中的所有不同蛋白质来发挥作用。一旦分离了这些蛋白质,就可以使用称为抗体的物质来检测特定的蛋白质。这些特定蛋白质的存在与否,或检测到的蛋白质的水平,将导致诊断。如果使用诊断性蛋白质印迹,则临床医生应要求测试,并使用可靠的实验室进行分析。

阅读蛋白质印迹结果

JIANCHACONGLINCHUANGYISHENGNEILIHUODEDEJIEGUO。GENJUSUOJIANCEDEGANRANHUOJIBINGDEBUTONG,WesternYINJIKENENGHUIBAOGAODUOGEBUTONGDETIAODAI,MEIGETIAODAIDEYANGXINGHUOYINXING。KENENGYOUXIEDANBAIZHITIAODAIBAOGAOLEDANBAIZHIYINJI,DANBINGBUBIAOSHIJIEGUO。QUEBAOLINCHUANGYISHENGTIGONGDEXINXIGAOZHINAXIEYINJISHI**。

XUNZHAOLEDUIDEDAXIAO。ZHEIXIEJIANGYOUSHUZIBIAOSHI,HOUGEN“ kDa”HUOQIANGEN“ p”。ZHEISHIYIJIANCEDAODEDANBAIZHIDEDAXIAO,BINGQIESHIDANBAIZHIYINJIFAZHONGFENLIDANBAIZHIDEGUIMO。ZHENGSHIZHEIXIEBUTONGBIANHAODETIAODAIDAIBIAOLEBUTONGDEDANBAIZHI,BINGJIANGJUEDINGSHIFOUCHANSHENGYANGXINGJIEGUO。

QUEDINGNAXIEPINDUANCHANSHENGJIJIJIEGUO,ZHEIKENENGYIWEIZHESHENME。LIRU,DUILAIMUBINGJINXINGJIANCESHI,YOUDUOGEBUTONGDETIAODAIKENENGHUICHANSHENGYANGXINGJIEGUO,ERQIZHONGRENHEYITIAODOUJIANGBIAOSHIYANGXINGJIEGUO。QITACESHIKENENGZHIHUICHANSHENGBUZHONGYAODEPINDAI。ZHEIXIEKENENGWEIBAOGAO,DANQINGQUEBAOQINGCHUNAXIESHIZHONGYAOBODUAN。XIANSHIYANGXINGJIEGUODETEDINGTIAODAIBIAOSHISUOJIANCEDEGANRANWEIYANGXINGJIEGUO。

YULINCHUANGYISHENGTAOLUNJIEGUOYIJIRENHEYILVHUOWENTI。YANGXINGJIEGUOKENENGXUYAOYIXIEZHILIAO,BINGQIECIJIEGUOHAIYINGYUDINGGOUWestern blotZHENDUANCESHIDELINCHUANGYISHENGTAOLUN。