排列3

电泳类型



电泳是基于不同分子具有与之相关的不同自然电荷这一事实在电场中分离生物分子的方法。这导致物质的不同成分在电场的影响下以不同的速率移动。想象一下,在一个托盘上有许多不同金属的小块,然后将磁铁放在托盘的一端。基于它们的特定电荷,不同的金属块(相当于“分子”)将以不同的程度被吸引到磁体上。本质上,这就是电泳中发生的事情,只是**初没有分离分子。

RUJIN,GEZHONGDIANYONGFANGFAZHENGZAISHIJIANZHONG。

电泳原理

DIANYONGGONGZUODEYUANYINGUIYINYUDIANCIWULIXUEZHONGDEYIGEJIBENFANGCHENGSHI:LIDENGYUDIANHECHENGYIGAIDIANCHUDEDIANCHANGQIANGDU。ZHEICAIYONGYIXIAXINGSHI:

F = qE

QIZHONGF =LI,q =DIANHE,E =DIANCHANGQIANGDU。

GAIFANGCHENGSHIBIAOMING,LIZISHANGDEDIANHEYUEGAO,SHIJIAJIDINGDIANCHANGCHANSHENGDELIYUEQIANG。ZHEIYIWEIZHEZHILIANGXIANGTONGDANDIANHEBUTONGDELIANGGELIZIJIANGZAIZHENGGECHANGZHONGYIBUTONGDESULVYIDONG。LINGWAI,RENHEDAIDIANFENZIYUNDONGDESUDUQUJUEYUQIDIANHEZHILIANGBI。ZHEIXIETEXINGHELIANXIZAIYIQI,SHIKEXUEJIAYOUKENENGJIANG**SHENGWUFENZIDECHENGFEN(LIRUHESUAN)FENLIWEIJIAOXIAODECHENGFEN。

凝胶电泳

SHIYONGLESANZHONGZHUYAOLEIXINGDENINGJIAODIANYONG。LIYONGMALINGSHUDIANFENKELIDEDIANFENNINGJIAODIANYONGSHIYIZHONGYIWU。ZAIQIONGZHITANGNINGJIAODIANYONGZHONG,SHIYONGCHUNHUADEDAFENZILIANGDUOTANGZUOWEIPEIYANGJI;ZHEITONGCHANGYONGYUDAXINGDNAFENZI。JUBINGXIXIANANNINGJIAODIANYONGSHI**CHANGJIANDELEIXING,YINWEITAFEICHANGWENDINGBINGQIEKEYIZAIHENDADEFENZINONGDUFANWEINEIGONGZUO。

不太常用的电泳仪类型

ZAIDADUOSHUSHIYANQINGKUANGXIA,**HAOJINXINGMOUZHONGXINGSHIDENINGJIAODIANYONG。QITACHANGJIANFANGSHIBAOKUOGAOFENBIANLVDIANYONG,MAOXIGUANDIANYONG,DENGDIANJUJIAO,**HUAXUEDIANYONG,ERWEIDIANYONGHEMAICHONGCHANGDIANYONG。

电泳设置的类型

 

DIANYONGYIYUSUOSHIYONGDETEDINGJIEZHIYOUHENDADEBUTONG。ZAIGUOQU,BIANJIEDIANYONGSHIBIAOZHUNFANGFA。ZAICISHIYANSHEZHIZHONG,JIANGCELIANGQIANYIFENZIZHENGGEBIANJIEDEYIDONGSULV。RUJIN,QUYUDIANYONGYUELAIYUEPUBIAN,FENZIQIANYIDAOYIXIAOKUAIZHISHANGDEBUTONGQUYUHUOQUYU。ZHEIBIBIANJIEDIANYONGGENGJUZHENDUIXING。**HOU,ZHIDIANYONGYOUSHIYONGYUXIAOFENZI。