排列3

聚丙烯酰胺凝胶电泳(PAGE)的原理

SDS-PAGE(JUBINGXIXIANANNINGJIAODIANYONG)SHIYIZHONGYONGYUGENJUDANBAIZHIHUNHEWUDEDAXIAOFENLIQICHENGFENDEFENXIFANGFA。

GAIJISHUJIYUZHEIYANGDEYUANLI,JIDAIDIANFENZIJIANGZAIDIANCHANGZHONGCHAOJUYOUXIANGFANFUHAODEDIANJIQIANYI。CHANGGUIDIANYONGJISHUBUNENGYONGYUQUEDINGSHENGWUFENZIDEFENZILIANG,YINWEIWUZHIZAININGJIAOZHONGDEQIANYILVQUJUEYUDIANHEHEDAXIAO。

WEILEKEFUZHEIGEWENTI,XUYAODUISHENGWUYANGPINJINXINGCHULI,YISHIQIHUODEJUNYUNDEDIANHE,RANHOUDIANYONGQIANYILVZHUYAOQUJUEYUCHICUN。DUIYUZHEIZHONGJUYOUBUTONGXINGZHUANGHEDAXIAODEDANBAIZHIFENZI,XUYAOJINXINGBIANXING(ZAISDSDEBANGZHUXIAWANCHENG),YISHIDANBAIZHISHIQUERJI,SANJIHUOSIJIJIEGOU。BEISDSFUGAIDEDANBAIZHIDAIFUDIAN,BINGZAISHANGYANGSHININGJIAOBINGZHIYUDIANCHANGZHONG,TAJIANGCHAOZHEYANGJI(DAIZHENGDIANDEDIANJI)QIANYI,BINGTONGGUOJIYUFENZISHAIFENZUOYONGDEDAXIAOFENKAI。TONGGUORANSE(DANBAIZHITEYIXING)JISHUJINXINGKESHIHUAHOU,KEYITONGGUOJIANGDANBAIZHIDEQIANYIJULIYUYIZHIFENZILIANGJIETI(BIAOJI)DEQIANYIJULIJINXINGBIJIAOLAIJISUANDANBAIZHIDEDAXIAO。